Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica

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Production of cell mass and eicosapentaenoic acid (EPA) in ultrahigh cell density cultures of Nannochloropsis sp. (Eustigmatophyceae)

This work attempts to identify growth conditions for maximal productivity of cell mass and of eicosapentaenoic acid (EPA) in ultrahigh cell density cultures of Nannochloropsis sp. Using flat plate reactors with a narrow (1–2 cm) light path and rigorous stirring exposed to high photon flux densities (1000–3000 μmol photons m−# s−"), record population densities (1±2–1±4¬10"! cells ml−") were obta...

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A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long‐chain polyunsaturated fatty acid production

Nannochloropsis oceanica is an oleaginous microalga rich in ω3 long-chain polyunsaturated fatty acids (LC-PUFAs) content, in the form of eicosapentaenoic acid (EPA). We identified the enzymes involved in LC-PUFA biosynthesis in N. oceanica CCMP1779 and generated multigene expression vectors aiming at increasing LC-PUFA content in vivo. We isolated the cDNAs encoding four fatty acid desaturases ...

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A Novel Halophilic Lipase, LipBL, Showing High Efficiency in the Production of Eicosapentaenoic Acid (EPA)

BACKGROUND Among extremophiles, halophiles are defined as microorganisms adapted to live and thrive in diverse extreme saline environments. These extremophilic microorganisms constitute the source of a number of hydrolases with great biotechnological applications. The interest to use extremozymes from halophiles in industrial applications is their resistance to organic solvents and extreme temp...

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Eicosapentaenoic acid (EPA) reduces cardiovascular events: relationship with the EPA/arachidonic acid ratio.

The clinical efficacy of fish oil and high-purity eicosapentaenoic acid ethyl ester (hp-EPA-E) for treating cardiovascular disease (CVD) has been reported. Fish oil contains saturated and monounsaturated fatty acids that have pharmacological effects opposite to those of ω3 fatty acids (ω3). Moreover, ω3, such as EPA and docosahexaenoic acid (DHA), do not necessarily have the same metabolic and ...

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Clinical and epidemiological study of eicosapentaenoic acid (EPA) in Japan.

Between 1978 and 1980, we investigated and compared the dietary intake of fish meat, platelet function, rheological properties of blood and mortality rate due to thrombotic cardiovascular diseases between the residents of fishing and farming villages. Notable finding is that the dietary intake of EPA in the residents of fishing village was nearly 3 times (2.5 g/day) higher than that (0.9g/day) ...

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ژورنال

عنوان ژورنال: Algal Research

سال: 2020

ISSN: 2211-9264

DOI: 10.1016/j.algal.2019.101766